I mean, look at the poor thing, all covered in tubes like it was some kind of science experiment.

.....

Wait a sec, it is.

Anyway, this one was still sealed with aluminum foil because it was just out of the autoclave.

We looked into the parts of the fermenter and its purposes and such. Here's a drawing:

The names of the parts basically tells the purpose already, doesn't it?

And here's an attempt to label a photo of the fermenter:

It's so much easier in 3D, I swear. OTL

After weighing... we poured 100mL of the LB medium into this HUGE shaker flask

ehum ehum...some medium had spilled onto the table..Oops!!!

2L of distilled water is not as light as what you think,ahha...coz...is super heavy to pour them into the flask !!!

Equipment Preparation (Day 1)

1. pH electrode was calibrated using standard buffer solution.
2. pH, pO2, foam and level probe was installed into the top plate.
3. Additional agent lines for acid, base and antifoam were connected.
4. Other accessories such as exhaust condensers, air inlet and exhaust filters were also installed.
5. Cables except temperature probe was disconnected
6. All silicone tubings were clamped except for exhaust filter and female STT coupling of sampling unit.
7. All filters and sockets were covered with aluminumfoil
8. Equipment was autoclaved with steam at 121ºC for 20 minutes.
9. pO2 probe was polarized for at least 6 hours and calibrated by aerating with nitrogen.
10. Additional lines were connected to peristaltic pumps.

Using distilled water to test how a fermenter works first.

Seed Culture Preparation (Day 1)

1. pGLO transformed E.coli was obtained and streaked onto a LB/Amp/Ara plate and incubated for 24hrs.

It glow under the UV light! Yea !!! Nice one !

Seed Culture Preparation (Day 2)

1. Several colonies of pGLO transformed E.coli from the LB/Amp/Ara plate was obtained and transferred to flask containing 100mL LB medium with ampicillin.
2. Flask was placed in shaking incubator and incubated at 32ºC for 24 hours.