NYP.// MB0704 \\. Bioprocess

[10 December 2008]- Objectives

MB0704 Proudly presenting the study of

Vinegar-making...

[Overall Objective]

To culture micro-organisms in batch fermenter and to grow

and extract green fluorescent protein(GFP)

Experiment No 1: Famililiarisation with the Bioreactor

and its Operation

1) To get familiarized with the parts and components of microbial

and mammalian bioreactors
2) To get introduced to the basic operation procedure of a bioreactor

Experiment No 2: Equipment,Media and Seed Culture Preparation

1) To be able to describe the steps to prepare a bioreactor
2) To prepare the media for seed culture and scale-up fermentation 3) To prepare seed culture for scale-up fermentation

Experiment No 3: Inoculation,Fermentation and Monitoring

1) To carry out scale-up fermentation process to increase the yield of desired

Experiment No 4: Isolation and Purification of Product

1) To be able to isolate and purify the product (desired protein)

0Comments

♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦